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The analytical techniques routinely used in Biochemistry take a very important place in Biochemistry, learning them is not just a requirement but, absolutely essential. This is because, the understanding of the subject mainly comes from the strong basis of the experiments and techniques on which the theories are built.This is 4 credit course. This course deals with various techniques their principles, experimental details etc., including general Laboratory techniques, separation techniques based on hydrodynamic properties, centrifugation (sub-cellular fractionation etc), and Chromatographic, and Electrophortic techniques , HPLC, Purification methods, ELISA etc. Application in biomedical research and some clinical diagnostic methods RIA, ELISA, PET etc., Moleular diagnostics ,PCR, etc., Microscopic techniques and clinical biochemistry.