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Indian Institute of Technology Kanpur

Cell Culture Technologies

Indian Institute of Technology Kanpur and NPTEL via Swayam


The course will be a short primer to understand how ‘animal cell culture technologies’ have strengthen the bio-medical research from basic research to the modern drug discovery. Animal cell culture was first performed in the very first decade of 19th century. Since then, tremendous development has taken place in this field. The lectures will help the researcher to appreciate the developments during last hundred years and will help them to independently set up cell culture laboratories. For non-biologist, it will be an informal way to demystify the intriguing routes of biomedical research where cell culture is a very ‘potent tool’.INTENDED AUDIENCE : UG and PG students pursuing biology, biotechnology, zoology and bio-engineering.PRE REQUISITE : Biology at standard 10th (Secondary school examination)INDUSTRY SUPPORT : Biomedical industries, Biotechnology industry, Drug -discovery industry


Week 1: Introduction & biology of cultured cellsWeek 2: Equipments, aseptic techniques, safety protocolsWeek 3: Culture vessels & media developmentWeek 4: Serum-free medium development & sterilizationWeek 5: Primary culture, secondary culture, cloning & selectionWeek 6: Cell separation, characterization, differentiation & transformationWeek 7: Contamination, cryo-preservation & cyto-toxicityWeek 8: Organo-typic culture & specialized cell culture techniques

Taught by

Prof. Mainak Das


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