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Indian Institute of Technology Bombay

Interactomics : Basics & Applications

Indian Institute of Technology Bombay and NPTEL via Swayam

Overview

Proteins are the key effectors of any living system and are largely responsible for the functioning of a cell. Intricate cell signaling and molecular triggers are dependent on interactions involving proteins at the cellular level. It is due to this very reason that, in an age where clinical biology is thriving to make an impact in global health-care and biomedical diagnostics, there has been a surge of interest in the area of Interactomics. Interactomics essentially involves the study of interactions between biomolecules, particularly proteins and the consequences of those interactions in a biosystem.Due to rapidly evolving technological platforms in biology, there is a need to keep pace with latest developments in field to explore their versatile applications. Interactions resulting from protein-protein, protein-peptide, protein-RNA, protein-DNA or protein- small molecule have immense application in life-sciences and translational biology. Through this course, we aim to provide an interface between distinguished scientists involved in interactomics research, industrial partners, faculties and students. This course would feature an intensive lecture series followed by some demonstrations designed to provide the much needed training required to explore the endless possibilities in interactomics research using genomics and proteomics approach, that can be useful for a researcher at any stage.Various technology platforms such as Protein microarrays, Label-free biosensors and Next-Generation Sequencing (NGS) Technology will be discussed. The students/participants will gain exposure to the above mentioned technologies and its applications from some of the experts in the field, from both- academia and industry perspective. INTENDED AUDIENCE : Biology or biotechnology background students having interest in latest technologies, (BE/B.Tech) Biotechnology Students, B.Sc. students science background but course is open to allPREREQUISITES : NoneINDUSTRY SUPPORT : GE Healthcare, Pall Life Sciences, Thermofisher Scientific, Illumina

Syllabus

Week 1- Interactomics: Basics and ApplicationsLecture 1: Introduction to ProteomicsLecture 2: Introduction to InteractomicsLecture 3: High throughput platforms of interactomics: Protein arraysLecture 4: Cell-free expression based protein microarraysLecture 5: NAPPA: Recombinational Cloning, Basic workflow, Surface Chemistry, Printing and Assessment
Week 2- Interactomics: Basics and ApplicationsLecture 6: NAPPA Technology and Protein Arrays-ILecture 7: NAPPA Technology and Protein Arrays-IILecture 8: Biomarkers: Harnessing the immune system for early detection of disease-ILecture 9: Biomarkers: Harnessing the immune system for early detection of disease-IILecture 10: Biomarkers: Harnessing the immune system for early detection of disease-III
Week 3- Interactomics: Basics and ApplicationsLecture11: NAPPA & its applications in study of antibody immune response in disease & in drug screening-ILecture12: NAPPA & its applications in study of antibody immune response in disease & in drug screening-IILecture13: NAPPA & its applications in study of antibody immune response in disease & in drug screening-IIILecture 14: Using functional proteomics to identify biomarkers and therapeutic targets-ILecture 15: Using functional proteomics to identify biomarkers and therapeutic targets-II
Week 4- Interactomics: Basics and ApplicationsLecture 16: Applications of protein microarrays in Malaria Research-ILecture 17: Applications of protein microarrays in Malaria Research-IILecture 18: Introduction to Bioprinting and Iris™ Optical QC Benefits-ILecture 19: Introduction to Bioprinting and Iris™ Optical QC Benefits-IILecture 20: Screening of autoantibody signatures in cancer patients: Lab demonstration
Week 5- Interactomics: Basics and ApplicationsLecture-21: Basics of Image Scanning and data acquisitionLecture-22: Applications of protein arrays in the identification of autoantibody signatures-ILecture-23: Applications of protein arrays in the identification of autoantibody signatures-IILecture-24: Applications of protein microarrays in deciphering PTMs and biological networksLecture-25: Basics and Applications of Reverse Phase Protein Arrays-I
Week 6- Interactomics: Basics and ApplicationsLecture-26: Basics and Applications of Reverse Phase Protein Arrays-IILecture-27: Basics and Applications of Reverse Phase Protein Arrays-IIILecture-28: An overview of label-free technologiesLecture-29: An overview of label-free technologiesLecture-30: Surface Plasmon Resonance- Principles and Assays-II
Week 7- Interactomics: Basics and ApplicationsLecture-31: Basics of SPR: Surface chemistryLecture-32: Basics of SPR: Experimental designLecture-33: Protein immobilization for protein-protein interaction studiesLecture-34: Protein-protein interaction study: Binding analysisLecture-35: Protein-protein interaction study: Kinetic analysis
Week 8- Interactomics: Basics and ApplicationsLecture-36: Use of SPR in unravelling domain motif interactions of proteasomal assembly chaperonesLecture-37: Protein-small molecule interaction study: Immobilization Immobilisation & binding analysisLecture-38: Protein-small molecule interaction study: Kinetic analysisLecture-39: An introduction to biolayer interferometry (BLI) and its applications in protein researchLecture-40: Biomolecular interactions using Bio-Layer Interferometry (BLI)-I
Week 9- Interactomics: Basics and ApplicationsLecture 41: Biomolecular interactions using Bio-Layer Interferometry (BLI)-IILecture 42: Lab session- An introduction to Bio-Layer Interferometry (BLI) and its applications in protein researchLecture 43: Applications of label-free technologies-IILecture 44: Biomolecular interaction analytics using MicroScale ThermophoresisLecture 45: Mass Spectrometry coupled Interactomics-I
Week 10- Interactomics: Basics and ApplicationsLecture 46: Mass Spectrometry coupled Interactomics-IILecture 47: Next-Generation Sequencing Technology- Ion Torrent™Lecture 48: NGS Technology - Bioinformatics and data analysis-ILecture 49: NGS Technology - Bioinformatics and data analysis-IILecture 50: Next-Generation Sequencing Technology- Illumina
Week 11- Interactomics: Basics and ApplicationsLecture 51: Agilent complete NGS target enrichment workflow for exomes, targeted panels and beyondLecture 52: The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome-ILecture 53: The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome-IILecture 54: Statistical Analysis-ILecture 55: Statistical Analysis-II
Week 12- Interactomics: Basics and ApplicationsLecture 56: Secondary Data AnalysisLecture 57: Pathway Enrichment and Network AnalysisLecture 58: Data Repositories and DatabasesLecture 59: Application of multi-omics approach for better understanding of cancersLecture 60: Integrated Omics and Systems Biology- Conclusion

Taught by

Prof. Sanjeeva Srivastava

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