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Swayam

Microbial Genetics

St. Xavier's College (Autonomous), Kolkata and CEC via Swayam

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Overview

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This course on Microbial genetics will have discussions on the different basic aspects of genetics spread over 34 modules. The course content will include genome organization and different types, advantages, applications of mutation and mutagens. It will cover different types, host range, curing, replication, purification, incompatibility of Plasmids and the mechanism of genetic exchange among microbes by recombination and the process of conjugation, transformation and transduction. The genetics of T4 bacteriophage and that of Transposable elements in both prokaryotic and eukaryotic systems would also be discussed.

Syllabus

Week 1Module 01: Plasmid 1Module 02: Plasmid 2Module 03: Plasmid 3Module 04: T4 bacteriophage

Week 2Module 05: Transposons and transposition in prokaryotesModule 06: Transposons and transpositions in eukaryotesModule 07: Application of transposons in modern biologyModule 08: Model of recombination
Week 3Module 9: Genetic analysis of recombination in bacteriaModule 10: Gene transfer in bacteria-transduction Part IModule 11: Types of transduction: Generalized transductionModules 12: Specialized transduction
Week 4
Module 13: Bacterial transformation IModule 14: Bacterial Transformation IIModule 15: Bacterial Conjugation IModule 16: Bacterial conjugation II

Week 5Module 17: Mutation: General introductionModule 18: Different types of mutationModule 19: Advantages and applications of mutationsModule 20: Mutagen I:Introduction to mutagen- General aspect

Week 6Module 21: Mutagen II: Physical mutagensModule 22: Mutagen III: Chemical mutagensModule 23: Mutagen IV: Biological mutagenModule 24: Mutagenesis

Week 7Module 25: Level/Effect of mutation: at DNA sequence and gene levelModule 26: Biochemical level of mutationsModule 27: Effects of mutationsModule 28: Level of mutation: at chromosome levelModule 29: Effect of mutation at molecular implication level causing disorders

Week 8Module 30:Reverse mutation—Back mutation and suppressor mutationModule 31:Experiments related to mutation and reversion and mutation repairModule 32:Transformation of E.coli (xl1-blue) cells by the plasmid DNA (puc-19)Module 33:Induction of Enzyme Activity in Bacteria: The Lac OperonModule 34: To learn the process of bacterial conjugation through the transfer of genes coding for antibiotic resistance

Taught by

Dr. Sudeshna Shyam Choudhury

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