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NPTEL

Cell Culture Technologies

NPTEL and Indian Institute of Technology Kanpur via YouTube

Syllabus

Introduction of Cell Culture Technology.
Philosophy and complexity in cell culture.
To grow the cell outside the body.
Cell cycle concept.
Dividing cells.
Biology of cell culture.
Layout(s) & design(s) of cell culture facility.
Precautions during designing the lab layout - I.
Precautions during designing the lab layout - II.
Precaution during designing the lab out - III.
State of the art facility in cell culture lab - I.
State of the art facility in cell culture lab - II.
Specialized facility in cell culture lab.
Interaction of cell and glass/polycorbonate surface - II.
Lecture 15_2017.
Poly D lysine deposition.
Surface chemical analysis.
Cell growth process.
Cell surface interface.
Cell culture substrate patterning.
Introduction of define system.
Mechanical dissociation of hippocampal tissue.
Rules for mechanical dissociation of tissue.
Drum molecule testing.
Adult hippocampal neuron dissociation.
Cell separation & In vitro myelination cell culture mode - I.
Cell separation & In vitro myelination cell culture mode - II.
Cell separation & In vitro myelination cell culture mode - III.
Cell separation & In vitro myelination cell culture mode - IV.
Cell separation & in vitro myelination cell culture mode - V.
Fluorescent assisted cell sorting.
Condition for regenerated cells.
Introduction of skeletal muscle cell culture.
Skeletal muscle cell culture.
Cardiac muscle cell culture.
Advance cell culture modules - I.
Advance cell culture modules - II.
Advance cell culture modules - III.
Advance cell culture modules - IV.
Advance cell culture modules - V.

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Cell Culture Technologies

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