Educator's Guide to Nanopore 16S: Flongle Flow Cell Loading

This 13-minute tutorial from DNA Learning Center demonstrates the process of preparing and loading a Flongle flow cell for Nanopore DNA sequencing. Learn the complete workflow including preparing flow cell loading solutions, properly loading the Flongle flow cell, adding necessary buffers, and adding the library for sequencing. The video is part of an educator-focused series designed to make Nanopore technology more accessible for classroom use, particularly for course-based undergraduate research experiences (CUREs). Follow along with the clearly organized sections as the instructor walks through each step of this critical procedure in the Nanopore sequencing workflow. The full protocol is available at the nanopore4edu.org website for additional reference.