Learn the detailed protocols and optimization techniques for performing Immunofluorescence (IF) and Immunohistochemistry (IHC) procedures in this comprehensive laboratory lecture. Master each critical step of these essential molecular biology visualization techniques, from sample preparation and antibody selection to staining procedures and troubleshooting common issues. Gain practical insights into optimizing experimental conditions to achieve reliable and reproducible results for both IF and IHC methods used in cellular and tissue analysis.
Immunofluorescence and Immunohistochemistry: Step-by-Step Guide and Optimization - Lecture 11
Overview
Syllabus
Lecture 11_Immunofluorescence IF and Immunohistochemistry IHC
Taught by
NPTEL-NOC IITM
Related Courses
-
Demonstration for Immunofluorescence Procedure - Do's and Don'ts
-
Introduction to Tissue Microarrays - Applications and Creation Methods
-
Tips, Tricks, and Optimization: A User's Guide to BOND RX and Chromogenic Multiplexing in Research
-
Combining RNAscope Technology with Protein Detection - A Multi-Omics Approach Using RNA-Protein Integrated Co-Detection Workflow
-
Optimizing Your Tissue Processing - Lessons from the Laboratory
-
Tissue Preparations and Handling for Multiplex Imaging in Spatial Biology Research
