Quantitative Super-Resolution Microscopy to Reveal Insights into Mesoscale Chromatin Organization

In this hour-long talk, Professor Lothar Schermelleh presents his research on using quantitative super-resolution microscopy to investigate mesoscale chromatin organization. Learn how interference-based linear structured illumination microscopy (SIM) enables multi-color volumetric super-resolution imaging of chromatin organization in mammalian cell nuclei. Discover how Schermelleh's group leveraged SIM's capability to resolve single molecules and complexes, quantifying their stoichiometry and interaction behaviors in specific cellular states. The presentation covers recent findings on functional chromatin organization at the mesoscale and cell cycle-dependent cohesion complex organization relevant to sister chromatid cohesion and loop extrusion. As a Professor of Bioimaging and Academic Director of the Micron Bioimaging Facility at Oxford University, Schermelleh brings expertise from over 80 high-profile publications and pioneering work in super-resolution microscopy applications. His research explores how mammalian genome activity relates to 3D nuclear organization and the interplay between biophysical forces, cohesin complex activity, and epigenetic memory in regulating cell-specific transcriptional programs during differentiation and pathological states.