This course covers the central dogma of molecular biology, including DNA replication, transcription, and translation. Students will learn about DNA polymerization, DNA methylation, RNA processing, and protein synthesis in prokaryotes. The teaching method includes detailed explanations and concepts, along with experiments such as DNA footprinting. This course is intended for individuals interested in gaining a basic understanding of molecular biology concepts and processes.
Overview
Syllabus
Central dogma (replication, transcription and translation).
DNA replication 1 | process of DNA duplication.
DNA polymerase proofreading.
DNA polymerization | nucleotide addition during DNA synthesis.
Clamp loader and sliding clamp.
DNA clamp loader and primase.
DNA polymerization by DNA polymerase 1.
DNA methylation in DNA replication regulation.
DNA Replication initiation.
Lagging strand synthesis.
Termination of DNA replication in E.coli.
DNA footprinting experiment.
DNase footprinting.
TRANSCRIPTION 01.
RNA polymerase detail.
RNA processing.
Protein synthesis in prokaryotes.
Protein synthesis process.
Lac operon concept class 12.
Lac operon repression - lac repressor.
Lac operon regulation.
DNA replication initiation in E coli.
DNA rolling circle replication.
Rolling circle replication advanced.
D loop replication.
Pan handle model of DNA replication.
Replication of single stranded plasmids.
Copy number control in plasmids.
Copy number regulation by cop genes.
Multiple copy number plasmids.
Low copy number plasmids.
Iteron mediated copy number control.
Copy number control of f plasmids.
Taught by
Shomu"s Biology
