Simultaneous Polyclonal Antibody Sequencing and Epitope Mapping by Cryo-Electron Microscopy and Mass Spectrometry

Join this one-hour webinar presented by Dr. Joost Snijder, who explores an innovative analytical approach for characterizing antigen-specific antibody repertoires directly from secreted proteins in convalescent serum. Learn how the combination of cryo-electron microscopy (cryo-EM) and mass spectrometry (LC-MS/MS) enables simultaneous antibody sequencing and epitope mapping. Discover the performance of the deep-learning tool ModelAngelo in determining de novo antibody sequences from reconstructed 3D volumes of antibody-antigen complexes, with potential accuracies of up to 80-90%. Understand how EM-derived templates improve MS-based sequencing accuracy in complex antibody mixtures and how publicly available EMPEM reconstructions can be leveraged for this approach. The webinar covers the significance of antibodies in adaptive immunity, the integration of cryo-EM and MS methods for comprehensive antibody characterization, and practical applications of deep-learning tools in immunological research.