Additionally, it will show you how to use genomic analysis tools to analyze whole genome sequencing data to detect resistance genes (and or other genes of interest) in a simple and easy way using online tools freely available.
In the new new version an additional module including detection of specific resistance mechanisms was added.
After this course you should be able to:
1. Describe the most important families of antimicrobials and mode of action
2. Understand the basic concepts of antimicrobial resistance from several perspectives (clinical, research and microbiological)
3. Enumerate and describe how bacteria can become resistant and the mechanisms that may be involved in that process
4. Describe how antimicrobial resistance emerges and spreads around the world including concepts of antimicrobial resistance transfer, selection and dissemination
5. Enumerate the methods used for antimicrobial susceptibility testing (AST)
6. Compare dilution and diffusion methods and know the basic techniques of agar disk diffusion, broth dilution and agar dilution methods
7. Have detailed theoretical knowledge on how to perform the main methods in a laboratory
8. Know the basic concepts about analysis and interpretation of results of AST, including different breakpoints, cut-off setting and their applications.
9. Understand the importance and related concepts related to quality management and quality assurance method standardization, applied to AST
10. Relate the information obtained in this course with real cases of resistant bacteria spreading in patients, the community, animals or the environment
11. Relate the phenotypical results with results from genotyping using molecular techniques for detection of resistance mechanisms
12. Understand the concept and be able to apply genomic analysis tools used to detect resistance genes and other relevant genes from Whole Genome Sequencing (WGS) data (with demonstration of selected online tools)
Disclaimer: Please note that the guidelines and methods referred or links included in these materials are updated when the videos lectures are produced and before the course is released, however these might become outdated with time.