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Blood Film Morphology - A Practical Guide

University of Western Australia via Coursera

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Overview

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This is a unique online course which teaches students the basics of blood film analysis. This course is specifically tailored to those with a background in Medical Science, Medicine or Pathology who are studying or starting work in a haematology laboratory but also serves as a valuable continuing education resource. To be successful in this course, access to a haematology laboratory is desirable but not essential. This is not a textbook or a laborious examination of every blood film abnormality but rather focusses on the approach – both an overall method for analysing a blood film, and a framework for analysing quantitative and qualitative changes in blood cells. At each step students are taught a method, provided with the theoretical framework for interpreting abnormalities, and shown examples of abnormalities highlighting their clinical significance. This is reinforced with frequent in-lecture interactive quizzes, end of lecture mcqs, and suggested lab exercises which engage the student with the laboratory and their colleagues. At the end of each module and at the end of the whole series are summative assessments which must be successfully completed to attain the course certificate.

Syllabus

  • Course Introduction
    • This 'Course Introduction' Module 1 describes the background to the blood film. It provides you with an overview of what takes place in a laboratory when a film of blood is analyzed. '1.1 - Course Overview' describes the background to the blood film. It explains how a FBC analyser works in '1.2 - The Analyser' and the potential sources of error in analyser results. It then explains which blood counts should trigger the making of a blood film and how that blood film is made in '1.3 - Importance of Making a Blood Film'. The '1.4 - A methodical approach to blood film analysis' lesson ends by suggesting and demonstrating a systematic method for blood film analysis. Module 1 ends with a graded 'End of Module 1 Quiz' for you to complete and a supplementary reading list.
  • Red Cell Morphology
    • This 'Red Cell Morphology' module takes you through a systematic method of examination of red cell morphology. It starts with a basic approach in '2.1 Red Cell Morphology Basics', then examines red cell quantity and red cell quality in '2.2 Describing Microcytic, Macrocytic and Normocytic Anaemias in blood films' and '2.3 Recognising and describing common poikilocoytes'. At each step, you are taught a method, provided with the theoretical framework for interpreting abnormalities, and shown examples of abnormalities highlighting their clinical significance in '2.4 Recognising and describing red cell inclusion'. 'The 'Red Cell Morphology' Module ends with a graded 'End of Module 2 Quiz' and a supplementary reading list.
  • White Cell Morphology
    • This 'White Cell Morphology' Module 3 takes you through a systematic method of examination of white cell morphology, following the template for blood film analysis in '3.1 A framework for analysing white cell quantitative disorders'. It starts by examining problems of white cell quantity, then examines the many important variants of white cell quality by using a a unique structured process for analysing an abnormal white cell population in '3.2 Analyzing an abnormal leukocyte population'. This takes you through the morphology of reactive white cells, lymphoproliferative diseases, leukaemias and myelodysplasia in '3.3 Analysing lymphoproliferative disorders', '3.4 Analysing acute leukaemias' and '3.5 Examining qualitative changes in white cells' . At each step you are taught a method, provided with the theoretical framework for interpreting abnormalities, and shown examples of abnormalities highlighting their clinical significance. You will be required to complete a grade End of Module 3 quiz and look up the supplementary reading list.
  • The 4Ps of Morphology - Platelets, Pregnancy, Paediatrics and Parasites
    • This '4Ps of Morphology - Platelets, Pregnancy, Paediatrics and Parasites' module deals with an assortment of important specific areas of blood film morphology. It first completes the systematic analysis of the blood film by discussing quantitative and qualitative changes in platelets in '4.1 Platelets (Thrombocytopenia & Thrombocytosis)'. In '4.2 Pregnancy', we then discuss the important physiologic and pathologic changes in the FBC and film during pregnancy. The '4.3 Paediatric' lecture emphasises that children are not just little adults by showing how the normal ranges for the FBC and normal appearance of films can differ at different ages, and then discussing a framework for morphologic diagnosis in paediatric anaemia, thrombocytopenia and pancytopenia. The '4.4 Parasite' lesson concludes with a review of malaria – demonstrating the lifecycle of the parasite, the morphologic appearance of different species, and a system to distinguish between different species. You are expected to complete a graded 'End of Module 4' quiz and look up the supplementary reading list.
  • Live Blood film analysis - Putting it all together
    • In this 'Live Blood Film Analysis - Putting it all together' module, we demonstrate the use of our method for blood film analysis using videos of real slide examinations for the five cases. The presentation format for each case is similar: each case starts by providing a case history and full blood count (FBC) parameters. You are encouraged to consider a differential diagnosis (DDx) for each case. We will follow the template for blood film analysis. Finally we arrive at a morphologic conclusion and ask you how you can help the clinician reach a diagnosis based on the blood film. The case studies are not assessed. However, viewing them is necessary to see how we apply our method and integrate the concepts taught in Module 1 - 4. As this is a module putting everything you have learned together in individual case studies, there will be no graded 'End of Module 5 quiz' for you to complete

Taught by

Matthew Wright

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4.9 rating at Coursera based on 61 ratings

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