This course on Getting Started in Cryo-EM aims to teach learners the fundamentals of Cryo-Electron Microscopy. By the end of the course, students will understand the basic anatomy of electron microscopes, Fourier transforms, image formation, sample preparation techniques, tomography, single-particle analysis, and 2D crystallography. The course uses a combination of lectures, review slides, and practical demonstrations to help learners grasp the concepts effectively. This course is intended for individuals interested in delving into the field of Cryo-Electron Microscopy, including students, researchers, and professionals in related scientific disciplines.
Overview
Syllabus
Getting Started in Cryo-EM with Professor Grant Jensen.
Welcome to Cryo-EM - G. Jensen.
Cryo-EM Review Slides URL.
Introduction: Why Electrons? - G. Jensen.
Part 1: Basic Anatomy of the EM - G. Jensen.
Part 1: Electron Guns - G. Jensen.
Part 1: EM Lenses - G. Jensen.
Part 1: The Column - G. Jensen.
Part 1: Sample Chamber - G. Jensen.
Part 1: Energy Filters - G. Jensen.
Part 1: Detectors - G. Jensen.
Part 1: Vacuum Systems - G. Jensen.
Part 1: Summary and Safety - G. Jensen.
Part 2: Fourier Transforms for Beginners - G. Jensen.
Part 2: 1-D Sine Waves and Their Sums - G. Jensen.
Part 2: 1-D Reciprocal Space - G. Jensen.
Part 2: 2-D Waves and Images - G. Jensen.
Part 2: 2-D Transforms and Filters - G. Jensen.
Part 2: 3-D Waves and Transforms - G. Jensen.
Part 2: Convolution and Cross-Correlation - G. Jensen.
Part 3: Image Formation - G. Jensen.
Part 3: Amplitude and Phase Contrast 2016 - G. Jensen.
Part 3: Wave Propagation and Phase Shifts - G. Jensen.
Part 3: The Contrast Transfer Function - G. Jensen.
Part 3: Defocus and Its Effects - G. Jensen.
Part 3: Envelopes - G. Jensen.
Part 3: CTF Correction - G. Jensen.
Part 4: Fundamental Challenges in Biological TEM - G. Jensen.
Part 4: Sample Prep: Room Temperature Methods - G. Jensen.
Part 4: Sample Prep: Methods Involving Freezing - G. Jensen.
Part 4: Sample Prep: Grids - G. Jensen.
Part 4: 3-D Reconstruction - G. Jensen.
Part 4: Dose Limitations - G. Jensen.
Part 5: Tomography - G. Jensen.
Part 5: Tomography Data Collection and Reconstruction - G. Jensen.
Part 5: Tomography Identfying Objects of Interest - G. Jensen.
Part 5: Tomography Limitations - G. Jensen.
Part 5: Tomography Parameters and Handedness - G. Jensen.
Part 6: Single Particle Analysis - G. Jensen.
Part 6: SPA Sample Prep - G. Jensen.
Part 6: SPA Data Collection - G. Jensen.
Part 6: SPA Reconstruction Basic Workflow - G. Jensen.
Part 6: SPA Reconstruction Additional Topics - G. Jensen.
Part 6: SPA Interpretations and Limitations - G. Jensen.
Part 7: 2D Crystallography Intro and Sample Prep - G. Jensen.
Part 7: FT of a 2D Crystal - G. Jensen.
Part 7: 2D Crystallography Data Collection and Reconstruction - G. Jensen.
Part 7: Helical Tubes - G. Jensen.
Part 7: Farewell - G. Jensen.
Taught by
caltech
