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Indian Institute of Technology Madras

Principles Of Downstream Techniques In Bioprocess

Indian Institute of Technology Madras and NPTEL via Swayam


The course covers the fundamentals, and design concepts of various down stream purification steps (unit operations) involved in a biochemical process. Down stream process is required to take a crude product from a fermentor or a bioreactor and purify it to the desired level. Hence it may involve solids, liquid and gas processing.The course covers cell breakage and recovery of intracellular material, Isolation of solids, Product recovery, Product enrichment/purification, Product polishing and finishing. This course is suitable for students pursuing their biotechnology, bioprocess engineering or other allied fieldThis course is also suitable for chemical engineers who would like to learn about separation techniques in biotechnology industries. The course will consist of lectures and solving problems. Problems will relate to design, estimating operating conditions and optimization of the process.

PREREQUISITES:Basics of biochemical engineering and thermodynamics



Week 1Lec-01 Introduction
Lec-02 Mass balance, Heat Balance, flow sheet
Lec-03 Costing
Week 2
Lec-04 Costing (continued), Physical and chemical principles in Downs stream
Lec-05 Problems in Mass balance, flow sheet
Lec-06 Cell Breakage
Week 3
Lec-07 Cell Breakage (continued)
Lec-08 Solid Liquid Separation
Lec-09 Solid Liquid Separation (continued)
Week 4
Lec-10 Solid Liquid separation-problems
Lec-11 Pre-treatment and Filters
Lec-12 Adsorption
Week 5
Lec-13 Adsorption (continued)
Lec-14 Adsorption (continued)
Lec15 Adsorption (continued)
Week 6
Lec-16 Liquid-Liquid Extraction
Lec-17 Liquid-Liquid extraction (continued)
Lec-18 Liquid-Liquid extraction (continued)
Week 7
Lec-19 Liquid-Liquid extraction (continued)
Lec-20 Reversed micellar and aqueous two phase extraction
Lec-21 Membranes
Week 8
Lec-22 Membranes (continued)
Lec-23 Membranes (continued)
Lec-24 Membranes (continued)
Week 9
Lec-25 Precipitation
Lec-26 Chromatography
Lec-27 Chromatography (continued)
Week 10
Lec-28 Chromatography (continued)
Lec-29 Chromatography (continued)
Lec-30 Chromatography (continued)
Week 11
Lec-31 Chromatography (continued)
Lec-32 Chromatography (continued)
Lec-33 Crystallisation
Week 12
Lec-34 Drying
Lec-35 Drying and Distillation
Lec-36 Future trends, Summary of the course

Taught by

Prof. Mukesh Doble

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