This introductory course in biophysical chemistry touches on some of the fundamentals of this field. Starting from basic elements in protein structure, the course covers other aspects like forces involved in protein folding, protein folding energy landscape, mechanisms of protein folding, mixing and relaxation techniques commonly used to study fast protein folding, and finally winds up with a discussion on some spectroscopic tools (Absorption, Fluorescence and IR) that are very popular in the field of proteins.
Overview
Syllabus
Intro.
A Course on Bio-physical Chemistry.
Protein Structure.
Secondary Structure of Proteins.
Secondary Structure of Proteins (Contd.).
Tertiary Structure.
Forces in Protein Folding.
Forces in Protein Folding (Contd.).
Electrostatics (Contd.).
Intermolecular Interactions.
Dipole-Dipole Interaction.
Electrostatics (Contd..).
Hydrophobic Effect.
Hydrophobic Effect (Contd.).
Hydrogen Bonding.
Protein Stability Curves.
Thermodynamics of Protein Unfolding.
Thermodynamics of Protein Unfolding (Contd.).
Mechanism of Chemical Denaturation.
Pressure Induced Denaturation (The P-T Diagram).
Protein Folding Pathways and Energy Landscapes.
Diffusion.
Diffusion (Contd.).
Diffusion (Contd..).
Langevin Equation and Brownian Motion.
Langevin Equation and Brownian Motion (Contd.).
Langevin Equation and Brownian Motion (Contd..).
Protein Folding : Mechanisms and Kinetics.
Protein Folding : Mechanisms and Kinetics (Contd.).
Protein Folding : Mechanisms and Kinetics (Contd..).
Protein Folding : The Chevron-Plot.
Protein Folding Kinetics : Rapid Mixing and Relaxation Techniques.
Protein Folding Kinetics : Rapid Mixing and Relaxation Techniques (Contd.).
Protein Folding Kinetics : Rapid Mixing and Relaxation Techniques (Contd..).
Protein Folding Kinetics : Rapid Mixing and Relaxation Techniques (Contd...).
Experimental Tools.
Spectroscopy : The Franck Condon Principle.
Spectroscopy : The Franck Condon Principle (Contd.).
Electronic Spectroscopy Absorption and Fluorescence.
Fluorescence..
Fluorescence Quenching.
Infrared Spectroscopy of Proteins.
Infrared Spectroscopy of Proteins (Contd.).
Taught by
IIT Delhi July 2018
