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Indian Institute of Technology, Kharagpur

Comprehensive Molecular Diagnostics and Advanced Gene Expression Analysis

Indian Institute of Technology, Kharagpur and NPTEL via Swayam

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ABOUT THE COURSE: Explore the frontiers of medical science with our course on "Molecular Diagnostics and Advanced Gene Expression Analysis." Uncover the historical evolution and advantages of molecular techniques, delve into the intricacies of nucleic acids, and master cutting-edge methodologies like PCR, NGS, and CRISPR-Cas9. Discover their clinical applications in infectious diseases, cancer, genetics, and more. Navigate the ethical considerations and future trends shaping the field. Join us to revolutionize your understanding of modern healthcare, molecular bioinformatics and precision medicine. INTENDED AUDIENCE: Medical Laboratory Professionals: This includes medical technologists, clinical laboratory scientists, and technicians who perform diagnostic tests. A course in molecular diagnostics can enhance their skills and knowledge in using advanced molecular techniques for disease diagnosis.Medical Practitioners: Doctors, especially those specializing in fields like oncology, infectious diseases, genetics, and personalized medicine, can benefit from understanding molecular diagnostic techniques to make more informed treatment decisions.Research Scientists: Researchers in academia, pharmaceuticals, and biotechnology can use molecular diagnostics to study diseases, develop new therapies, and conduct clinical trials. A course can equip them with the necessary skills to incorporate molecular diagnostics into their work.Genetic Counselors: Professionals who provide information and support to individuals and families with genetic disorders can expand their expertise by learning about molecular diagnostics and its applications.Pharmaceutical Professionals: Those working in the pharmaceutical industry can benefit from understanding molecular diagnostics for drug development, pharmacogenomics, and monitoring treatment responses.Public Health Officials: Professionals involved in disease surveillance, outbreak investigation, and epidemiology can utilize molecular diagnostics for accurate and rapid disease detection.Biomedical Engineers: Individuals working in the field of medical device development and instrumentation can apply molecular diagnostics principles to design innovative diagnostic tools.Healthcare Administrators: Administrators responsible for managing healthcare facilities and services can benefit from understanding the potential impact of molecular diagnostics on patient care and resource allocation.Graduate and Postgraduate Students: Students pursuing degrees in medical sciences, biotechnology, genetics, microbiology, or related fields can enhance their knowledge and increase their employability by taking a course in molecular diagnostics.Entrepreneurs and Innovators: Individuals interested in starting healthcare-related businesses, such as diagnostic laboratories or biotech startups, can gain valuable insights into the field through a molecular diagnostics course. PREREQUISITES: Basics in molecular biology, Foundations in cell biology, biochemistry and biotechnologyINDUSTRY SUPPORT: Healthcare Sector/institutions, Biotechnology and Biosciences based companies, Research institutions, Pharmaceutical companies, diagnostic laboratories, Medical device manufacturers, Genetic testing laboratories.


Week 1: Basic Concepts including Central Dogma in Molecular Biology Definition and Scope of Molecular Diagnostics and Historical Developments Importance and advantages of molecular diagnostics over traditional methods, Nucleic Acid Structure and Function, DNA Replication and Repair, RNA Transcription and Processing, Protein Synthesis from mRNA-Translation
Week 2:
Tools of Molecular diagnostics and Gene expression Analysis (I) PCR (Polymerase Chain Reaction) Fundamentals, RT PCR and qPCR, Modifications of PCR-Hot start, Touch down, nested PCR, Multiplex, Modifications of PCR 2-Long-range PCR,Single-cell PCR,Fast-cycling PCR, Methylation-specific PCR (MSP), Digital Droplet PCR-modern implications, PCR-based mutation analysis
Week 3:
Tools of Molecular diagnostics and Gene expression Analysis (II) Principles (Sanger sequencing, Overview of NGS Technologies and Platform, Application of NGS in Molecular Diagnostics, Clinical Interpretation of NGS Data, Whole genome vs Whole exome sequencing, Targeted gene panels, NGS library preparations)
Week 4:
Tools of Molecular diagnostics and Gene expression Analysis (III) DNA Microarray, FISH (Fluorescence in situ Hybridization), Serial analysis of gene expression, RNA sequencing, Tiling array, DNA protein interaction- chromatin immune precipitation.
Week 5:
Techniques of Gene Manipulation: RNA interference and detection methods, Recombinant DNA Technology, CrispR-CAS9 technology, Epigenetics and diseases, DNA methylation analysis.
Week 6:
Proteomics: Clinical Applications Overview of proteomics techniques and workflows, Protein separation techniques-brief discussion of gel electrophoresis and
chromatography, mass spectrometry, label-free and isotope labelling methods, role of metabolomics in laboratory diagnosis.
Week 7:
Proteomics: Advanced topics in Clinical Proteomics High throughput proteomics like-Shotgun and data independent acquisition (DIA), Single cell proteomics and spatial profiling, methods to detect post translational modification and protein-protein interaction, proteomic data analysis and bioinformatic tools, Luminex multiplex assays and its application in biomarker analysis.
Week 8:
Molecular Diagnostics in Infectious Diseases Syndromic Panels and Multiplex Assay, Molecular identification of Microorganism- covering bacterial, viral, fungal and parasitic diseases, antimicrobial resistance testing, POC Molecular diagnostics for infectious diseases, Molecular diagnostics in Hospital acquired infections.
Week 9:
Molecular Diagnostics in Cancer Management Cancer markers, Liquid biopsies in cancer detection, circulating Tumour DNA (ctDNA) analysis, Monitoring treatment response with molecular diagnostics, Molecular diagnostics in targeted therapy, Digital PCR, Molecular diagnostics quality control
Week 10:
Molecular Diagnostics in Genetic and Inherited Disorders Genetic testing and inherited diseases, Non-Invasive Prenatal testing (NIPT) and reproductive genetics, Molecular diagnostics in rare genetic disorders, Pharmacogenomics and Personalized Medicine, genetic counselling and patient education.
Week 11:
Molecular Diagnostics in Medicine Molecular diagnostics in Metabolic disease, Molecular diagnostics in Neurodegenerative disease, Molecular diagnostics in Respiratory, Molecular diagnostics in Gastrointestinal disorders, Molecular diagnostics in Endocrine disorders, Molecular diagnostics in Autoimmune disorders, Molecular diagnostics in Cardiovascular diseases, Molecular diagnostics in Transplantation diseases
Week 12:
Molecular Diagnostics: Quality control and Ethical Concerns in and Futuristic Trends Quality control in molecular diagnostics , Ethical Concerns in Molecular Diagnostics,Microfluidics and Lab-on-chip in molecular diagnostics, AI and ML in molecular diagnostics, Nanotechnology based molecular diagnostics, Single cell Analysis, Integration of Multi-omics Data.

Taught by

Prof. Aritri Bir, Prof. Arindam Ghosh



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