The course covers the fundamentals, and design concepts of various down stream purification steps (unit operations) involved in a biochemical process. Down stream process is required to take a crude product from a fermentor or a bioreactor and purify it to the desired level. Hence it may involve solids, liquid and gas processing.
The course covers cell breakage and recovery of intracellular material, Isolation of solids, Product recovery, Product enrichment/purification, Product polishing and finishing. This course is suitable for students pursuing their biotechnology, bioprocess engineering or other allied fields.
This course is also suitable for chemical engineers who would like to learn about separation techniques in biotechnology industries. The course will consist of lectures and solving problems. Problems will relate to design, estimating operating conditions and optimization of the process.
Lec-02 Mass balance, Heat Balance, flow sheet
Lec-04 Costing (continued), Physical and chemical principles in Downs stream
Lec-05 Problems in Mass balance, flow sheet
Lec-06 Cell Breakage
Lec-07 Cell Breakage (continued)
Lec-08 Solid Liquid Separation
Lec-09 Solid Liquid Separation (continued)
Lec-10 Solid Liquid separation-problems
Lec-11 Pre-treatment and Filters
Lec-13 Adsorption (continued)
Lec-14 Adsorption (continued)
Lec15 Adsorption (continued)
Lec-16 Liquid-Liquid Extraction
Lec-17 Liquid-Liquid extraction (continued)
Lec-18 Liquid-Liquid extraction (continued)
Lec-19 Liquid-Liquid extraction (continued)
Lec-20 Reversed micellar and aqueous two phase extraction