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Indian Institute of Technology Madras

Downstream Processing

Indian Institute of Technology Madras and NPTEL via Swayam

This course may be unavailable.


The course covers the fundamentals, and design concepts of various down stream purification steps (unit operations) involved in a biochemical process. Down stream process is required to take a crude product from a fermentor or a bioreactor and purify it to the desired level. Hence it may involve solids, liquid and gas processing. The course covers cell breakage and recovery of intracellular material, Isolation of solids, Product recovery, Product enrichment/purification, Product polishing and finishing. This course is suitable for students pursuing their biotechnology, bioprocess engineering or other allied fields. This course is also suitable for chemical engineers who would like to learn about separation techniques in biotechnology industries. The course will consist of lectures and solving problems. Problems will relate to design, estimating operating conditions and optimization of the process.


Week 1

 Lec-01 Introduction

 Lec-02 Mass balance, Heat Balance, flow sheet

 Lec-03 Costing

Week 2

Lec-04 Costing (continued), Physical and chemical principles in Downs stream

Lec-05 Problems in Mass balance, flow sheet

Lec-06 Cell Breakage

Week 3

Lec-07 Cell Breakage (continued)

 Lec-08 Solid Liquid Separation

 Lec-09 Solid Liquid Separation (continued)

Week 4

Lec-10 Solid Liquid separation-problems

Lec-11 Pre-treatment and Filters

 Lec-12 Adsorption

Week 5

Lec-13 Adsorption (continued)

 Lec-14 Adsorption (continued)

 Lec15 Adsorption (continued)

Week 6

 Lec-16 Liquid-Liquid Extraction

Lec-17 Liquid-Liquid extraction (continued)

 Lec-18 Liquid-Liquid extraction (continued)

Week 7

Lec-19 Liquid-Liquid extraction (continued)

Lec-20 Reversed micellar and aqueous two phase extraction

 Lec-21 Membranes

Week 8

Lec-22 Membranes (continued)

Lec-23 Membranes (continued)

Lec-24 Membranes (continued)

Week 9

 Lec-25 Precipitation

 Lec-26 Chromatography

 Lec-27 Chromatography (continued)

Week 10

Lec-28 Chromatography (continued)

Lec-29 Chromatography (continued)

 Lec-30 Chromatography (continued)

Week 11

Lec-31 Chromatography (continued)

 Lec-32 Chromatography (continued)

 Lec-35 Crystallisation

Week 12

Lec-36 Drying

 Lec-37 Drying and Distillation

Lec-40 Future trends, Summary of the course



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