This course covers a wide range of biological techniques and tools, including chromatography, microscopy, nucleic acid hybridization, mass spectrometry, and proteomics. By the end of the course, students will be able to perform protein purification, DNA sequencing, and comparative genomic mapping. The teaching method includes theoretical explanations, practical demonstrations, and hands-on exercises. This course is intended for students and professionals in the field of biology who want to enhance their practical skills and knowledge in biological research techniques.
Overview
Syllabus
CHROMATOGRAPHY PART 1.
CHROMATOGRAPHY PART 2.
Chromatogram.
Gas chromatography.
HPLC chromatography principle and instrumentation.
Affinity chromatography.
Protein purification | protein dialysis.
Fluorescence loss in photobleaching or FLIP.
Fluorescence recovery after photobleaching | FRAP.
Gel filtration chromatography.
Hydropathy plot.
Ion exchange chromatography.
Isopycnic centrifugation.
Mobility of phospholipid.
Zonal ultracentrifugation.
Numerical aperture of a microscope.
Resolution of a microscope.
Polarizing microscopy.
Phase contrast microscopy.
Yeast two hybrid system.
Aberration of microscopic lenses | chromatic aberration, spherical aberration and coma effect.
X ray crystallography basics explained.
Protein tagging.
Isoelectric focusing in 2d gel electrophoresis.
Hybridoma.
Dark field microscopy.
DIC microscopy.
FRET - fluorescence resonance energy transfer (fret).
Nucleic acid hybridization | DNA hybridization.
Serological tests.
Nucleotide probe hybridization.
Nucleotide probe hybridization.
Restriction endonuclease.
Restriction endonuclease.
Restriction fragment length polymorphism.
Restriction fragment length polymorphism.
Sanger sequencing method of DNA | chain termination method.
Centrifugation.
Mass spectrometry part 5: Graph analysis.
AFLP.
RAPD.
Spectral karyotyping.
Comparative genome mapping.
Comparative genomic hybridization.
Microdisection of chromosome.
Metabolomics.
Proteomics.
Trypsin digestion.
Mass spectrometry part 9 time of flight analyzer.
Mass spectrometry part 8 quadruple analyzer.
Mass spectroscopy part 7 MALDI and ESI.
Mass spectroscopy part 6 Ionization in Mass spec.
Metastable ion.
Silver staining.
Mclafferty rearrangement problems 2.
Mclafferty rearrangement problems 1.
Mclafferty rearrangement.
2 D gel electrophoresis.
Flow Cytometry.
Taught by
Shomu"s Biology
