This course introduces to the basic biology of proteins and the new advanced science called as proteomics which aims to look into the protein properties from a global perspective, i.e., not undertaking one protein at a time, but an entire set of proteins in the milieu. The course will cover in detail the two major aspects of proteomics i.e., Gel-based proteomics and Mass spectrometry-based proteomics. The gel-based module will cover different techniques like SDS-PAGE, 2-DE, 2D-DIGE etc. These techniques had a major contribution in transition from protein chemistry to proteomics. Mass spectrometry, on the other hand, is an advanced analytical technique for accurate mass measurement. In this module, we will discuss the basics of mass spectrometry, sample preparations, liquid chromatography, hybrid mass spectrometers and quantitative proteomics techniques such as iTRAQ, SILAC and TMT using mass spectrometry. The course will also provide the basic knowledge about sample preparation, mass spectrometry workflow, different chromatography technologies and quantitative proteomics.INTENDED AUDIENCE :It would be applied to B.Sc., M.Sc. and MS.PREREQUISITES : Any B.Sc. Or M.Sc.The target audiences of this course are required to have a basic introduction to biology.
Week 1 : Basics of Proteins and Proteomics
Lecture 1 : Introduction to amino acids Lecture 2 : Introduction to Proteins Lecture 3 : Protein folding & misfolding Lecture 4 : Introduction to Proteomics Lecture 5 : Lab session – Protein-protein interaction using label-free biosensors Week 2 : Gel-based proteomics
Lecture 6: Sample preparation and pre-analytical factors Lecture 7 : Sample preparation: Pre-analytical factors (contd.) Lecture 8 : Sample preparation: Protein extraction and quantification Lecture 9 : One-dimensional electrophoresis Lecture 10 : Introduction to 2-DE Week 3 : Two-dimensional gel electrophoresis (2-DE)
Lecture 11 : 2-DE: Second dimension, staining & destaining Lecture 12 : 2-DE: Gel analysis Lecture 13 : 2-DE Applications Lecture 14 : 2-DE Applications (contd.) & Challenges Lecture 15 : Lab session - Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR & data analysis Week 4 : Difference in gel electrophoresis (DIGE) & Systems Biology
Lecture 16 : 2D-DIGE: Basics Lecture 17 : 2D-DIGE: Data analysis Lecture 18 : 2D-DIGE: Applications Lecture 19 : Systems biology and proteomics – I Lecture 20 : Systems biology and proteomics - II Week 5 : Basics of mass spectrometry
Lecture 21 : Fundamentals of mass spectrometry Lecture 22 : Chromatography technologies Lecture 23 : Liquid chromatography Lecture 24 : Mass spectrometry: Ionization sources Lecture 25 : Mass spectrometry: Mass analyzers Week 6 : Basics of mass spectrometry and sample preparation