This course on fluorescence spectroscopy aims to introduce learners to the limitations and possibilities of using fluorescence as a sensitive method for measuring compounds. The course covers topics such as the principles of spectroscopy, fluorescence, factors affecting the fluorescence signal, instrumentation, and ways to measure fluorescence. By the end of the course, learners will have a better understanding of fluorescence spectroscopy and its applications. The course is suitable for individuals interested in analytical chemistry, spectroscopy, or those working in fields where fluorescence spectroscopy is utilized.
Overview
Syllabus
Intro
Electromagnetic spectrum
What happens? Example: ketone
Molecular spectroscopy
Principles of spectroscopy
Principles of fluorescence
Tryptophan fluorescence
Fluorescence spectroscopy
Internal relaxation
Fluorescence dictionary - Part 11
Varian Eclipse
Xenon flash lamp
Instrumentation - PMT detector
Fluorophores - Molecular structure
Flourophores
Factors affecting the fluorescence signal
Concentration - Ideal conditions
Inner filter effect
Problem with the correction
Environment - Solvent
Environment - Temperature
Environment - Denaturant
Dynamic quenching
Static quenching
Non-radiative energy transfer
Scatter
Ways to measure fluorescence - Polarization
Ways to measure fluorescence - Time-decay
Fluorescence summary
Why fluorescence?
Options of measuring fluorescence
Second Order Advantage - PLS VS. PARAFAC
Proteins and salt solutions
Taught by
Chemometrics & Machine Learning in Copenhagen
