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Overview
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This webinar provides an introductory overview of how samples are prepared for sequencing by laboratories and how bioinformaticians use the resulting data to answer questions of interest using next-generation sequencing technologies. The course aims to explain the fundamental differences in NGS datasets and does not require prior background in laboratory techniques or bioinformatics. The teaching method includes conceptual examples, and the intended audience includes individuals interested in genomic technologies and bioinformatics.
Syllabus
Intro
Dr Sarver Introduction
Agenda
Historical Context
Sequence Read Archive
Data Sources
Laboratory Analysis
Sample Preparation
Library Preparation
Sequencing
Ligation
Sequencers
Sequencing Multiple Samples
Fast Queue Format
Quality checks
QC fermentation report
Mapping
IGV
Variant Calling
Annotation
General Conceptual Overview
Differential Expression
Liquid Biopsy
Conclusion
Questions
Sanger vs capillary sequencing
What is used as a human genome
Variant calling in interpretation
Allele dropout
Taught by
Ambry Genetics