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Molecular Biology

CEC and Gujarat University via Swayam


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The current core course has been specifically planned for the students studying in the arena of Zoology subject at College or University level as per the UGC approved curriculum. In addition, this particular core course can also be adopted by undergraduate students studying in the relevant disciplines of Biological Sciences encompassing Life-Science, Biochemistry, Biotechnology, Genetics, Biomedical Technology, Forensic Science, Pharmaceutical Sciences, Cancer Biology, Clinical Research, Microbiology, Bioinformatics, Paramedical as well as Medical fields.

The present core course has been organized magnificently to cover all the essential aspects related to Molecular Biology field. The course will provide a brief overview of Nucleic acid background comprising of salient features and models of DNA and RNA. The course will mainly focus on the study of principal molecular events of cell incorporating DNA Replication, Transcription and Translation in prokaryotic as well as eukaryotic organisms. The course will also emphasize Post Transcriptional Modifications and Processing of Eukaryotic RNA covering the concepts of Split genes, Introns, Exons, Splicing Mechanisms and RNA Editing. The course will also impart detailed explanation of Prokaryotic and Eukaryotic Transcriptional Regulation along with mechanism of Gene Silencing. During this course, the students will be imparted comprehensive understanding about key concept of DNA Repair Mechanisms. In addition, the course will provide glimpse of Ribo-switches, RNA Interference, miRNA and siRNA as a part of different types of Regulatory RNAs. The course will also provide the additional knowledge regarding various aspects of molecular biology in the form of supplementary study material.

Course Objectives:

The structure of the present core course on Molecular Biology has been magnificently designed with the perspective to achieve following key objectives:

To provide comprehensive background of Salient features of Nucleic Acids and DNA model to the course learners.

To impart detailed understanding of key events of molecular biology comprising of mechanism of DNA Replication, Transcription and Translation in Prokaryotes and Eukaryotes.

To provide adequate knowledge about Post Transcriptional Modifications andProcessing of Eukaryotic RNA to the course learners.

To give detailed explanation of Transcriptional Regulation with examples of lac operon and tryptophan operon in prokaryotic as well as eukaryotic organisms along with key concept of Gene Silencing to the course learners.

To develop comprehensive understanding regarding DNA Repair Mechanisms in the course learners.

To provide glimpse of types of Regulatory RNAs by imparting adequate informationabout Ribo-switches, RNA Interference, miRNA and siRNA to the course learners.

Eligibility forEnrollment:

Thosestudentswho have passed(10+2)withBiological Sciencesubject with at least55% marksand/or at present studying Zoology at undergraduate level.

Course Pre-requisite:

Pre-requisite for admission in this course will be(10+2)with anyBiological Sciencewith at least55% marks.

Intended Audience:
The course has been intended for thestudents studying in the arenas of relevant biological science disciplines encompassing Life-Science, Biochemistry, Biotechnology, Genetics, Bioinformatics, Microbiology, Forensic Science, Environmental Science, Biomedical Technology, Cancer Biology, Clinical Research, Pharmaceutical Sciences, Cancer Biology, Medical Science and Paramedical fields.
Reading Materials:
References and Web links have been providedin all the modules.

Learning Outcomes:

After successful accomplishment of the course, the learners will be able to acquire better understanding and comprehensive knowledge regarding most of the essential aspects of Molecular Biology subject which in turn will provide a fantastic opportunity to develop professional skill related to the field of molecular biology.



Unit-1: Nucleic Acids
Lecture 1: Salient Features of DNA and RNA• Lecture 2: Watson & Crick DNA Model• Lecture 3: Structural Organization of DNA• Lecture 4: DNA Estimation Unit-2: DNA Replication
Lecture 5: Central Dogma of Molecular Biology• Lecture 6: Prokaryotic DNA Replication• Lecture 7: Eukaryotic DNA Replication
Unit-3: Transcription

Lecture 8: Prokaryotic & Eukaryotic Transcription Unit-4: Translation
Lecture 9 :Genetic Code & Wobble Hypothesis
Lecture 10:Prokaryotic Translation
Lecture 11: Eukaryotic Translation
Lecture 12: Post Translational Modifications & Protein Synthesis Inhibitors
Unit-5: Post Transcriptional Modifications and Processing of Eukaryotic RNA
Lecture 13: Eukaryotic Post Transcriptional Modifications & RNA Processing
Unit-6: Gene Regulation
Lecture 14: Gene Regulation in Prokaryotes & Eukaryotes
Unit-7: DNA Repair Mechanism
Lecture 15: DNA Repair
Unit-8: Regulatory RNAs
Lecture 16: Types of Regulatory RNAs

Supplementary Study Material
Supplementary Lecture 17: Genome Information & Sequence Analysis• Supplementary Lecture 18: DNA Isolation• Supplementary Lecture 19: DNA Estimation : Part-1• Supplementary Lecture 20: DNA Estimation : Part-2

Taught by

Dr. Nayan K. Jain



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