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Online Course

Interactomics : Basics and Applications

Indian Institute of Technology Bombay and NPTEL via Swayam

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Found in Biology Courses

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Overview

Proteins are the key effectors of any living system and are largely responsible for the functioning of a cell. Intricate cell signaling and molecular triggers are dependent on interactions involving proteins at the cellular level. It is due to this very reason that, in an age where clinical biology is thriving to make an impact in global health-care and biomedical diagnostics, there has been a surge of interest in the area of Interactomics. Interactomics essentially involves the study of interactions between biomolecules, particularly proteins and the consequences of those interactions in a biosystem.   Due to rapidly evolving technological platforms in biology, there is a need to keep pace with latest developments in field to explore their versatile applications. Interactions resulting from protein-protein, protein-peptide, protein-RNA, protein-DNA or protein- small molecule have immense application in life-sciences and translational biology. Through this course, we aim to provide an interface between distinguished scientists involved in interactomics research, industrial partners, faculties and students. This course would feature an intensive lecture series followed by some demonstrations designed to provide the much needed training required to explore the endless possibilities in interactomics research using genomics and proteomics approach, that can be useful for a researcher at any stage.   Various technology platforms such as Protein microarrays, Label-free biosensors and Next-Generation Sequencing (NGS) Technology will be discussed. The students/participants will gain exposure to the above mentioned technologies and its applications from some of the experts in the field, from both- academia and industry perspective. INTENDED AUDIENCE : Biology or biotechnology background students having interest in latest technologies, (BE/B.Tech) Biotechnology Students, B.Sc. students science background but course is open to all PREREQUISITES : None INDUSTRY SUPPORT : GE Healthcare, Pall Life Sciences, Thermofisher Scientific, Illumina

Syllabus

COURSE LAYOUT Week 1 : Introduction to Interactomics and Protein Arrays, High throughput platforms of interactomics: Protein arrays, An introduction to Cell-free protein synthesis, Cell-free synthesis based protein microarrays: PISA and NAPPA, Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays Week 2 : Digging deeper into NAPPA: Basic Workflow, Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment, NAPPA Technology and Protein Arrays-I, NAPPA Technology and Protein Arrays-II, Biomarkers: Harnessing the immune system for early detection of disease-I Week 3 : Biomarkers: Harnessing the immune system for early detection of disease-II, Biomarkers: Harnessing the immune system for early detection of disease-III, NAPPA and its applications in study of antibody immune response in disease and in drug screening-I, NAPPA and its applications in study of antibody immune response in disease and in drug screening-II, NAPPA and its applications in study of antibody immune response in disease and in drug screening-III Week 4 : Using functional proteomics to identify biomarkers and therapeutic targets-I, Using functional proteomics to identify biomarkers and therapeutic targets-II, Conventional label based detection techniques for Protein microarrays, Basics of microarray image scanning Software for Image scanning and data processing Week 5 : Microarray Data Analysis: Part I, Microarray Data Analysis: Part II, Statistical Analysis-I, Statistical Analysis-II, Novel detection techniques for Protein microarrays Week 6 : Recombinational cloning and its application for Protein microarrays, Introduction to Bioprinting and Iris™ Optical QC Benefits-I, Introduction to Bioprinting and Iris™ Optical QC Benefits-II,Application of cell free expression protein microarrays in biomarker discovery, Application of protein microarray in biomarker discovery-I Week 7 : Application of protein microarray in biomarker discovery-II Applications of protein microarrays in Cancer Research-I, Applications of protein microarrays in Cancer Research-II, Application of cell free expression protein microarrays in immunological studies, Applications of protein microarrays in Malaria Research-I Week 8 : Applications of protein microarrays in Malaria Research-II, Basics and Applications of Reverse Phase Protein Arrays-I, Basics and Applications of Reverse Phase Protein Arrays-II, Basics and Applications of Reverse Phase Protein Arrays-III, Antibody signatures defined by high-content peptide microarray analysis Week 9 : Demo sessions/ Quiz practice/Application based expts, An overview of label-free technologies-I, An overview of label-free technologies-II, Surface Plasmon Resonance- Principles and Assays-I Surface Plasmon Resonance- Principles and Assays-II Week 10: Basics of SPR: Experimental design, Lab session– Basics of SPR: Surface chemistry, Protein immobilization for protein-protein interaction studies, Protein-protein interaction study: Binding analysis Protein-protein interaction study: Kinetics analysis Week 11: Use of SPR in unravelling domain motif interactions of proteasomal assembly chaperones Protein-small molecule interaction study: Immobilization & binding analysis,Protein-small molecule interaction study: Kinetic analysis,SPR: Interactive Session - I, SPR: Interactive Session - II Week 12: An overview of ellipsometry and interferometry techniques, Biomolecular interactions using Bio-Layer Interferometry (BLI)-I, Biomolecular interactions using Bio-Layer Interferometry (BLI)-II Lab session- An introduction to BioLayer Interferometry (BLI) and its applications in protein research, Kinetic analysis of protein-protein interaction using BLI

Taught by

Prof. Sanjeeva Srivastava

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