The course covers the learning outcomes and goals of understanding nuclear mechanics and dynamics in plant development, specifically focusing on root hair nucleus morphodynamics using a microfluidic approach. The individual skills taught include analyzing nuclear shape changes, studying the nucleo-cytoplasmic interphase, and utilizing microfluidic devices for experimental procedures. The teaching method involves a webinar series presentation by Marie-Edith Chabouté. The intended audience for this course is individuals interested in plant biology, cellular mechanics, and microfluidic techniques.
Real Time Tracking of Root Hair Nucleus Morphodynamics Using a Microfluidic Approach
Society for Experimental Biology via YouTube
Overview
Syllabus
Intro
Nuclear mechanics and dynamics in plant development
Nuclear shape changes in plant development
Mechanical stress reaching the nucleus?
Importance of the plant nucleo-cytoplasmic interphase
Change in nuclear shape starts beyond a threshold
Morphodynamics and mechanics of nucleus are reversible
Nucleus as a rheostat in plant development
Root hair (RH): a perfect model to study nuclear dynamics
Coverslip based microfluidic device (CMD)
Arabidopsis RH analysis: a microfluidic approach
Experimental procedure
Nuclear dynamics during tip growth
Nuclear dynamics: changes in nuclear shape during tips
Nuclear dynamics during RH development
First conclusions
Further exploration of microfluidic approaches
Nuclear mechano-transduction in plant development
Acknowledgements
Taught by
Society for Experimental Biology
